sirtuin fluorometric assay kits  (Biomol GmbH)

Journal: Frontiers in Aging

Article Title: Tissue-specific effects of bacterial PncA overexpression on NAD + metabolism and aging in mice: implications for tissue-specific aging interventions

doi: 10.3389/fragi.2025.1546017

Figure Lengend Snippet: PncA Increases the NAD + Level and Alleviates Liver Aging (a) Expression level of the PncA gene in the liver after AAV-PncA infection (n = 5 for each group, ****p < 0.0001) (b) Identification of nicotinamide activity in homogenized liver tissue samples (n = 5 for each group, ****p < 0.0001) (c) Measurement of NAD + levels following PncA overexpression in middle-aged mice (n = 5 for each group, ***p < 0.001) (d) qPCR-based mitochondrial quantification (n = 5 for each group, *p < 0.05) (e) Expression levels of genes associated with aging and mitochondrial function (n = 5 for each group, **p < 0.01) (f) NAD + levels in young mice with vector expression, aged mice with vector expression, and aged mice with PncA overexpression (n = 5 for young and aged mice with PncA overexpression group, n = 4 for aged mice group **p < 0.01) (g) Levels of NAD + -related metabolites in non-targeted metabolomic data (n = 3 for each group. ****p < 0.0001, **p < 0.01) (h) Western blot analysis of p16 Ink4a , p53, and SIRT1 proteins linked to aging and mitochondrial function in aged mouse livers with PncA overexpression (i) Effect of PncA overexpression on SIRT1 protein activity (n = 3 for each group, *p < 0.05) (j) Major metabolic pathways enriched with metabolites affected by PncA in non-targeted metabolomic data (k) Analysis of oil red staining in the liver and the statistical outcomes related to lipid droplets. Scale bars 500 µm.

Article Snippet: The SIRT1 Fluorometric Assay Kit (Elabscience, E-BC-F056) was employed to quantify SIRT1 activity following the manufacturer’s protocol.

Techniques: Expressing, Infection, Activity Assay, Over Expression, Plasmid Preparation, Western Blot, Staining

Journal: Cardiovascular Toxicology

Article Title: Pregnancy and Postpartum Effects of Electronic Cigarettes on Maternal Health and Vascular Function in the Fourth Trimester

doi: 10.1007/s12012-025-09961-x

Figure Lengend Snippet: Dams and litters characteristics (Mean ± SE)

Article Snippet: The level of Sirtuin 1 (SIRT1) was assessed from cerebral cortex homogenates obtained from ~ 200 mg of tissue and application of 100 mg of total protein per well using an ELISA kit (NOVUS Biologicals, Catalog #NBP2-80,302) according to the manufacturers’ protocol.

Techniques: Clinical Proteomics